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Image Search Results
Journal: Scientific Reports
Article Title: Glyoxal-derived advanced glycation end products (GO-AGEs) with UVB critically induce skin inflammaging: in vitro and in silico approaches
doi: 10.1038/s41598-024-52037-z
Figure Lengend Snippet: GO-AGEs enhance the degradation of extracellular matrix by increasing the matrix metalloproteinase release while decreasing expression of COL1A and SIRT1 in NHDF cells. ( A ) MTT assay showing cytotoxic effects of GO-AGEs (100 μg/mL) in the presence or absence of UVB irradiation (125 mJ/cm 2 ), along with the control group in NHDF cells. ( B , C ) ELISA results demonstrating the MMP-1 ( B ) and COL1A ( C ) levels in the conditioned medium of NHDF cells upon treatment with GO-AGEs (100 μg/mL) in the presence or absence of UVB irradiation (125 mJ/cm 2 ) along with the control group. ( D ) Western blot analysis showing the expression level of MMP-1, COL1A, and SIRT1 proteins after treatment with GO-AGEs (100 μg/mL) in the presence or absence of UVB irradiation (125 mJ/cm 2 ) along with the control group. ( E – G ) Quantification of the expression levels of MMP-1 ( E ), COL1A ( F ), and SIRT1 ( G ). Each value represents the mean ± SEM of triplicate experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the control group and # p < 0.05 and ### p < 0.001 vs. the UVB treatment group induced by UVB irradiation (125 mJ/cm 2 ).
Article Snippet: After 48 h of incubation, the MMP-1 and COL1A concentrations were analyzed using
Techniques: Expressing, MTT Assay, Irradiation, Enzyme-linked Immunosorbent Assay, Western Blot
Journal: Scientific Reports
Article Title: Glyoxal-derived advanced glycation end products (GO-AGEs) with UVB critically induce skin inflammaging: in vitro and in silico approaches
doi: 10.1038/s41598-024-52037-z
Figure Lengend Snippet: Molecular docking analysis of methylglyoxal-lysine dimer (MOLD) with different target proteins performed by the AutoDock Vina program. The complex structures of MOLD with IL-1β ( A ), IL-6 ( B ), TNF-α ( C ), COX2 ( D ), P65 ( E ), RAGE ( F ), MMP-1 ( G ), COL1A ( H ), SIRT1 ( I ).
Article Snippet: After 48 h of incubation, the MMP-1 and COL1A concentrations were analyzed using
Techniques: